Faculty Research 1970 - 1979

Title

The genetics and developmental regulation of L-glycerol 3-phosphate dehydrogenase.

Document Type

Article

Publication Date

1975

Keywords

Alleles, Animal, Chromatography-DEAE-Cellulose, Chromosome-Mapping, Crosses-Genetic, Electrophoresis, Genes, Genotype, Glycerolphosphate-Dehydrogenase: bi, Heat, Isoenzymes: bi, Kidney: en, Liver: en, Mice, Mice-Inbred-Strains, Muscles: en, Myocardium: en, Organ-Specificity, Protein-Denaturation, Species-Specificity, SUPPORT-U-S-GOVT-P-H-S

JAX Source

J-Cell-Physiol. 1975 Apr; 85:437-47.

Abstract

In addition to reviewing the genetic regulation of L-glycerol 3-phosphate dehydrogenase during development in the mouse, new evidence is presented that the electrophoretic properties of L-glucerol 3-phosphate dehydrogenase in Mus castaneus are determined by an allele (d) at the Gdc-1 locus. Accordingly there are three alleles at the Gdc-1 locus; the b allele in C57BL/6J mice differs from the d allele in electrophoretic properties and the c allele in BALB/cJ mice differs from the d allele with respect to both heat denaturation and electrophoretic properties. Identical segregation patterns of the L-glycerol 3-phosphate dehydrogenase phenotypes in liver, kidney, and skeletal muscle from offspring of an F2 generation produced from parents with the c/d genotype suggest that the Gdc-1 locus is the major structural locus for L-glycerol 3-phosphate dehydrogenase in these tissues. Heart muscle was pooled from mice of the F2 generation with either c/c or d/d genotypes at the Gdc-1 locus as determined by analysis of liver L-glycerol 3-phosphate dehydrogenase. The chromatographic properties of L-glycerol 3-phosphate dehydrogenase from the heart muscle was determined on DEAE-cellulose ion exchange columns. The elusion profile of the L-glycerol 3-phosphate dehydrogenase indicates that the Gdc-1 locus is also the major structural locus in heart muscle.

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