Faculty Research 1970 - 1979

Title

Ly-4.2: a cell membrane alloantigen of murine B lymphocytes. I. Population studies.

Document Type

Article

Publication Date

1975

Keywords

Antibody-Specificity, Antigen-Antibody-Reactions, Antilymphocyte-Serum, B-Lymphocytes: im, Bone-Marrow: im, cy, Cell-Membrane: im, Chromium-Radioisotopes, Complement, Cytotoxicity-Tests-Immunologic, Genotype, Isoantibodies, Isoantigens, Lymph-Nodes: cy, Mice, Neoplasms-Experimental: im, Peyer's-Patches: cy, SUPPORT-U-S-GOVT-P-H-S, Thymus-Gland: im, cy, Trypan-Blue

JAX Source

J-Immunol. 1975 Feb; 114():848-55.

Abstract

The Ly-4 locus defines an alloantigenic specificity present on the surface of lymphocytes of some mouse strains and detected in lymphocytotoxicity tests by an antiserum (BALB/c times SWR)F1 anti-B10.D2. In this paper are presented results which indicate that Ly-4.2 is predominantly represented on B cells and not T cells. In cytotoxicity tests with rabbit complement, the distribution of Ly-4.2 is restricted: thymus 5%, TDL 12%, spleen 60%, lymph node 30%, bone marrow 36%, Peyer's patches 55%, peritoneal exudate cells 35%, and peripheral lymphocytes 35%. This distribution is the reciprocal of that obtained with anti-Thy-1 antiserum. Further, when both reagents were used together, almost all cells in these tissues were lysed, excepting those with bone marrow. When anti-Ly-4.2 and anti-Thy-1.2 were used sequentially, it was apparent that these specificities were present on different cell populations. As Thy-1 is a marker for T cells, Ly-4 is therefore, presumably, a marker for B cells. This conclusion was confirmed by testing mice depleted of T cells ("nude:; thymectomy + anti-lymphocyte serum treatment; thymectomy, irradiation + reconstitution with bone marrow) where the majority of lymphocytes were Ly-4.2+, Thy-1.2-; and by testing tumors, where, for the most part, this reciprocal relationship held true. Appropriate studies indicate that the Ly-4.2 antibody does not detect a surface immunoglobulin allotype. Anti-Ly-4.2 anti-serum may provide a useful alloantigenic marker distinct from immunoglobulin, mouse-specific bone marrow-derived lymphocyte anti;en, and "beta: for detecting some or all, B cells.

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