Faculty Research 1970 - 1979

Title

Egasyn, a protein complexed with microsomal beta-glucuronidase.

Document Type

Article

Publication Date

1975

Keywords

Electrophoresis-Polyacrylamide-Gel, Glucuronidase: im, me, Goats: im, Immunoglobulins-Fab, Isoenzymes: im, me, Lysosomes: en, Macromolecular-Systems, Mice, Mice-Inbred-Strains, Microsomes-Liver: en, Molecular-Weight, Mutation, Precipitin-Tests, Protein-Binding, Proteins: me, SUPPORT-U-S-GOVT-P-H-S

JAX Source

J Biol Chem 1975 Feb 10; 250(3):1146-8.

Abstract

Two polypeptide chains are present in murine beta-glucuronidase precipitated with a specific anti-beta-glucuronidase antibody F(ab)2 fragment. One is the catalytic subunit of beta-glucuronidase and the other has the properties predicted for the hypothetical beta-gluronidase membrane anchor protein. The new protein, named egasyn, is associated with microsomal, but no lysosomal beta-glucuronidase. It is released from the microsomal beta-glucuronidase complex by heat treatment. The YBR strain of mice carrying the Eg degrees mutation does not form an egasyn-beta-glucuronidase complex and is unable to retain beta-glucuronidase on microsomal membranes.

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