Faculty Research 1970 - 1979
Defined nutrient medium for the in vitro maintenance of Xenopus laevis oocytes.
Document Type
Article
Publication Date
1976
Keywords
Cell-Membrane: ul, Cells-Cultured, Chromosomes: ul, Culture-Media, Endocytosis, Female, Oocytes: de, me, ul, Ovum: ul, Progesterone, Proteins: bi, RNA: bi, SUPPORT-U-S-GOVT-NON-P-H-S, Vitellogenin: me, Xenopus
First Page
418
Last Page
427
JAX Source
In-Vitro. 1976 Jun; 12(6):418-27.
Abstract
A procedure is described for the isolation and culture of large numbers of follicle cell-free Xenopus laevis oocytes in all stages of development. The isolation procedure involves the incubation of pieces of ovary in a calcium-free solution OR2 containing 0.2% collagenase. A defined nutrient medium for the maintenace of the oocytes in vitro is presented. It is shown that this medium, referred to as DNOM, can maintain certain morpological and functional characteristics of oocytes for periods up to 3 weeks.
Recommended Citation
Eppig JJ,
Dumont JN.
Defined nutrient medium for the in vitro maintenance of Xenopus laevis oocytes. In-Vitro. 1976 Jun; 12(6):418-27.