MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes.

Authors

C Labarca
K PaigenFollow

Document Type

Article

Publication Date

1977

Keywords

Catalysis, Chromatography-Affinity, Electrophoresis-Polyacrylamide-Gel, Female, Glucuronidase: bi, ge, In-Vitro, Mice, Mice-Inbred-A, Oocytes: en, Ovum: en, RNA-Messenger: ge, ph, SUPPORT-U-S-GOVT-NON-P-H-S, Translation-Genetic, Xenopus

First Page

4462

Last Page

4465

JAX Source

Proc Natl Acad Sci U S A 1977 Oct; 74(10):4462-5.

Abstract

Catalytically active mouse beta-glucuronidase (beta-D-glucuronide glucuronosohydrolase, EC 3.2.1.31) is formed when Xenopus oocytes are injected with mouse RNA enriched for poly(A)-containing mRNA sequences. With the RNA from androgen-induced kidneys, the efficiency of translation is comparable to that of endogenous Xenopus messenger, and the fidelity of translation is high. Detection of glucuronidase messenger by formation of a catalytically active product is several orders of magnitude more sensitive than detection by incorporation of isotopically labeled amino acids. As well as providing a sensitive technique for examining the regulation of gene expression, the system makes available an opportunity to study the regulation of post-translational polypeptide processing of a lysosomal enzyme.

Recommended Citation

Labarca C, Paigen K. MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes. Proc Natl Acad Sci U S A 1977 Oct; 74(10):4462-5.