Faculty Research 1970 - 1979
Comparison of benzo[a]pyrene metabolism by human peripheral blood lymphocytes and monocytes.
Document Type
Article
Publication Date
1978
Keywords
Chromatography-High-Pressure-Liquid, Comparative-Study, Epoxy-Compounds, Flavones, Human, Hydrocarbons-Chlorinated, In-Vitro, Lymphocytes: de, me, Monocytes: de, me, Naphthalenes, SUPPORT-U-S-GOVT-P-H-S
First Page
261
Last Page
268
JAX Source
Cancer Lett 1978 Nov; 5(5):261-8.
Abstract
Here we report a more sensitive high-pressure liquid chromatographic (HPLC) assay of benzo[a]pyrene (BP) metabolites elaborated by human monocytes and lymphocytes using only 10 X 10(6) cells for HPLC analysis. The major metabolites formed by both lymphocytes and monocytes were 3-hydroxy-BP, 9-hydroxy-BP and quinones. BP-dihydrodiols were also found by HPLC analysis, the major one being BP-7,8-dihydrodiol for both cell types. In addition, a peak slightly more polar than 9,10-dihydrodiol was formed by lymphocytes. The 7,8-diol peak was eliminated by the addition of 1,1,1-trichloropropene oxide to the incubation mixture. The presence of alpha-napthoflavone resulted in an overall decrease in metabolite production.
Recommended Citation
Vaught JB,
Gurtoo HL,
Paigen B,
Minowada J,
Sartori P.
Comparison of benzo[a]pyrene metabolism by human peripheral blood lymphocytes and monocytes. Cancer Lett 1978 Nov; 5(5):261-8.