Faculty Research 1970 - 1979

Title

Cytotoxic T-cell responses in mice infected with influenza and vaccinia viruses vary in magnitude with H-2 genotype.

Document Type

Article

Publication Date

1978

Keywords

Cross-Reactions, Cytotoxicity-Immunologic, Genes-MHC-Class-II, Genotype, H-2-Antigens: ge, Immunologic-Memory, Influenza: im, Mice, Orthomyxovirus-Type-A-Human: im, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes: im, Vaccinia: im, Vaccinia-Virus: im

JAX Source

J Exp Med 1978 Aug 1;148(2):534-43

Abstract

Secondary effector T-cell populations generated by cross-priming with heterologous influenza A viruses operate only in H-2K or H-2D compatible situations, when assayed on SV40-transformed target cells infected with a range of influenza A viruses. The H2-Kb allele is associated with a total failure in the generation of influenza-immune cytotoxic T cells, though this is not seen for the primary response to vaccinia virus. In both influenza and vaccinia development of effector T cells operating at H-2Db is greatly depressed in B10.A(2R) (kkkddb) and B10.A(4R) (kkbbbb), but not in B10 (bbbbbb), mice. However, there is no defect in viral antigen expression at either H-2Kk or H-2Db in B10.A(2R) target cells. This apparently reflects some inadequacy in the stimulator environment, as (A/J X B6) F1 T cells can be induced to respond at H-2Db when exposed to vaccinia virus in an irradiated B6 but not in a B10.A(4R) recipient. The present report, together with the accompanying paper by Zinkernagel and colleagues, records the first rigorous demonstration of both a nonresponder situation and a probable Ir-gene effect for conventional infectious viruses. Possible implications for the evolution of H-2 polymorphism and mechanisms of Ir gene function are discussed.