A comparison between oocyte growth in coculture with granulosa cells and oocytes with granulosa cell-oocyte junctional contact maintained in vitro.

Authors

J J. EppigFollow

Document Type

Article

Publication Date

1979

Keywords

Carbon-Dioxide: me, Cells-Cultured, Comparative-Study, Female, Granulosa-Cells: ph, In-Vitro, Intercellular-Junctions: ph, Leucine: me, Mice, Oocytes: gd, me, Ovum: gd, Pyruvates: me, SUPPORT-U-S-GOVT-NON-P-H-S, Uridine: me

First Page

345

Last Page

353

JAX Location

43,221

JAX Source

J-Exp-Zool. 1979 Aug; 209(2):345-53.

Abstract

Evidence is presented that strongly supports the hypothesis that the junctional association between oocytes and granulosa cells must be maintained to promote oocyte growth and development in vitro and that the coculture of oocytes with granulosa cells is not a sufficient condition for oocyte development. Furthermore, it is shown that incorporation of uridine and leucine by oocytes into TCA-insoluble material is significantly greater in granulosa cell-enclosed oocytes than in cocultured oocytes.

Recommended Citation

Eppig JJ. A comparison between oocyte growth in coculture with granulosa cells and oocytes with granulosa cell-oocyte junctional contact maintained in vitro. J-Exp-Zool. 1979 Aug; 209(2):345-53.