Faculty Research 1980 - 1989
A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies.
Document Type
Article
Publication Date
1988
Keywords
Antibody-Specificity, Blotting-Southern", Cell-Line, Drug-Resistance, Horseradish-Peroxidase, Human, Hybridomas, alpha-Fetoproteins
First Page
913
Last Page
915
JAX Location
see Journal collection
JAX Source
Mol Immunol 1988 Sep; 25(9):913-915.
Abstract
A metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant one (multidrug resistance). Tetradomas were selected in the presence of two selective agents: aminopterin and actinomycin D. Using this approach we produced tetradomas secreting bispecific MAbs to horseradish peroxidase and human alpha-fetoprotein.
Recommended Citation
Chervonsky AV,
Faerman AI,
Evdonina LV,
Jazova AK,
Kazarov AR,
Gussev AI.
A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies. Mol Immunol 1988 Sep; 25(9):913-915.