Faculty Research 1990 - 1999

Title

Effects of granulocyte/macrophage colony-stimulating factor on the development and differentiation of CD5-positive macrophages and their potential derivation from a CD5-positive B-cell lineage in mice.

Document Type

Article

Publication Date

1998

Keywords

Antigens-CD5: me, B-Lymphocytes: cy, me, Bone-Marrow-Cells: ph, Cell-Aging: de, ph, Cell-Differentiation: de, ph, Cell-Line, Coculture, Granulocyte-Macrophage-Colony-Stimulating-Factor, Immunophenotyping, Liver: em, cy, Macrophages: de, me, ul, Mice, Mice-Inbred-BALB-C, Mice-Inbred-C3H, Peritoneal-Cavity: cy, Stromal-Cells: ph, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Time-Factors

JAX Source

Am J Pathol 1998 Feb;152(2):445-56

Grant

CA20408/CA/NCI

Abstract

In co-cultures of either the murine pre-B cell line J13, fetal liver cells, or adult peritoneal or bone marrow cells with ST2 mouse bone marrow stromal cells in the presence of granulocyte/macrophage colony-stimulating factor (GM-CSF), the development of CD5+ macrophages was demonstrated by immunohistochemical staining and flow cytometry. Although CD5+ macrophages were not present in the peritoneal cavities of normal mice, approximately 30% of the peritoneal macrophages in viable motheaten (mev/mev) mice, deficient in SHP-1 protein tyrosine phosphatase, expressed cell surface CD5 and B220, markers for B cells. In the mev/mev mice, GM-CSF level in peritoneal fluid was increased significantly. At 5 days after daily intravenous injection with GM-CSF, many CD5+ macrophages appeared in the peritoneal cavity and in omental milky spots of normal mice but fewer in osteopetrosis (op) mutant mice, deficient in macrophage (M)-CSF. These results indicate that GM-CSF, in combination with M-CSF, induces the development and differentiation of CD5+ macrophages in the peritoneal cavity, particularly in the omental milky spots of mice. In the peritoneal cavity of GM-CSF-treated mice, the percentages of hematopoietic progenitor cells doubly positive for CD5 and CD34 or c-kit and of macrophage precursor cells doubly positive for CD5 and ER-MP58 or ER-MP20 were increased significantly during the development of CD5+ macrophages and CD5 B cells, suggesting that CD5+ macrophages and B cells may share a bipotential progenitor in vivo.

Please contact the Joan Staats Library for information regarding this document.

Share

COinS