Faculty Research 1990 - 1999

Title

Aberrant regulation of bone trace elements in motheaten and osteopetrosis mutant mice.

Document Type

Article

Publication Date

1998

Keywords

Animal, Bone-and-Bones, Comparative-Study, Immunologic-Deficiency-Syndromes, Macrophage-Colony-Stimulating-Factor Mice, Mice-Inbred-C3H, Mice-Inbred-C57BL, Mice-Knockout, Mice-Mutant-Strains, Osteoclasts-metabolism, Osteopetrosis, Protein-Tyrosine-Phosphatase, Receptors-Macrophage-Colony-Stimulating-Factor, Trace-Elements, Zinc

JAX Source

Cell Mol Biol (Noisy-le-grand) 1998 Mar;44(2):315-9

Grant

CA20408/CA/NCI

Abstract

Increasing numbers of genetic diseases involving bone development and models for these diseases have been identified recently. Analysis of these bone diseases have revealed that regulated action of multiple growth factors and subsequent signal transduction are essential for normal bone formation. In this paper, two murine mutant mice viable motheaten and osteopetrosis are analyzed. Mice with the recessive 'viable motheaten' mutation express a severe immunodeficiency syndrome and bone defects. Mutations at the motheaten locus were shown to be the result of aberrant splicing of the gene encoding hematopoietic cell phosphatase (Hcph). Mice homozygous for the osteopetrosis mutation develop congenital osteopetrosis due to a severe deficiency of osteoclasts. It has been recognized that bone trace element composition analysis helps to define bone-related physiological conditions. We have analyzed bone trace element composition in viable motheaten and osteopetrosis mutant animal models in this study. In order to gain insights into the effects of particular genetic defects on bone trace element composition, inductively coupled plasma atomic emissions spectrometry (ICP-AES) analysis was performed. Marked changes in bone trace element levels were found in limb bones of viable motheaten and osteopetrosis mutant mice. An assessment of these trace element spectrum in the two mutant models with respect to each genetic defects are discussed in this paper.

Please contact the Joan Staats Library for information regarding this document.

Share

COinS