Faculty Research 1990 - 1999

Title

Differential recognition of MBP epitopes in BALB/c mice determines the site of inflammatory disease induction.

Document Type

Article

Publication Date

1998

Keywords

Alternative Splicing/immunology Amino Acid Substitution, Animal, Antibody Specificity, Clone Cells, Cloning, Molecular, Demyelinating Diseases/immunology, Epitopes/immunology Exons/genetics/immunology, Female, Mice, Mice, Inbred BALB C, Mice, Neurologic Mutants, Molecular Sequence Data, Myelin Basic Proteins/genetics/immunology, Neuritis/immunology, Peptide Fragments/genetics/immunology, Peripheral Nervous System/immunology, Receptors, Antigen, T-Cell/analysis/immunology, T-Lymphocytes/chemistry/immunology

JAX Source

J Neuroimmunol 1998 Aug 14;89(1-2):73-82

Grant

NS31152/NS/NINDS, NS32441/NS/NINDS

Abstract

Although myelin basic protein (MBP)-recognizing T cells are not readily obtained after immunization of BALB/c mice with MBP (reflecting the BALB/c resistance to actively induced experimental autoimmune encephalomyelitis (EAE)), they can be expanded and cloned after several rounds of in vitro culture. The majority of BALB/c-derived clones recognize an epitope defined by MBP peptide 59-76. When transferred to naive BALB/c recipients, these clones cause classical EAE, with characteristic inflammation and demyelination of the central nervous system (CNS). We previously showed that two related clones recognizing a minor epitope, defined by MBP peptide 151-168, cause inflammation and demyelination preferentially of the peripheral nervous system (PNS). Because MBP has alternatively spliced isoforms, residues 151-168 are not present contiguously in all MBP isoforms. In order to determine whether induction of PNS disease is idiosyncratic to these sister clones, or related to their properties of epitope recognition, an independent T-cell line with similar recognition properties was studied. Clone 116F, derived from a BALB/c shiverer mouse, expresses a different T-cell receptor (TCR), with distinct TCR contact residues, but like the previously described T cells, this clone requires residues from both exons 6 and 7 for optimal stimulation. When adoptively transferred to BALB/c recipients, this clone preferentially induces disease of the PNS. A control BALB/c shiverer-derived MBP 59-76-recognizing clone, in contrast, induces CNS disease. These data strongly suggest that the site of disease initiation may correlate with epitope recognition, particularly when alternative isoforms are involved.

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