Faculty Research 1990 - 1999

Title

Spectrin localization in osteoclasts: immunocytochemistry, cloning, and partial sequencing.

Document Type

Article

Publication Date

1998

Keywords

Blotting-Western, Chick-Embryo, Cloning-Molecular, DNA-Complementary, Immunohistochemistry, Microscopy-Confocal, Microscopy-Immunoelectron, Osteoclasts: me, ul, Spectrin: ge, me, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

J Cell Biochem 1998 Nov 1;71(2):204-15

Grant

S07RR07161/RR/NCRR, AR42091/AR/NIAMS, DE04345/DE/NIDR

Abstract

The presence of spectrin was demonstrated in chick osteoclasts by Western blotting and light and electron microscopic immunolocalization. Additionally, screening of a chick osteoclast cDNA library revealed the presence of alpha-spectrin. Light microscope level immunocytochemical staining of osteoclasts in situ revealed spectrin staining throughout the cytoplasm with heavier staining found at the marrow-facing cell margin and around the nuclei. Confocal microscopy of isolated osteoclasts plated onto a glass substrate showed that spectrin encircled the organelle-rich cell center. Nuclei and cytoplasmic inclusions were also stained and the plasma membrane was stained in a nonuniform, patchy distribution corresponding to regions of apparent membrane ruffling. Ultracytochemical localization showed spectrin to be found at the plasma membrane and distributed throughout the cytoplasm with especially intense staining of the nuclear membrane and filaments within the nuclear compartment.

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