Faculty Research 1990 - 1999

Title

Utilization of microhomologous recombination in yeast to generate targeting constructs for mammalian genes.

Document Type

Article

Publication Date

1998

Keywords

Animal, Base-Sequence, Chimera, Cloning-Molecular, DNA-Primers, Exons, Gene-Targeting, Genetic-Vectors, Genomic-Library, Mammals, Mice, Mice-Inbred-Strains, Molecular-Sequence-Data, Mutagenesis, Polycystic-Kidney-Diseases, Polymerase-Chain-Reaction, Recombination-Genetic, Saccharomyces-cerevisiae, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Transformation-Genetic

JAX Location

see Journal Stacks

JAX Source

Mutat Res 1998 Jun; 401(1-2):11-25.

Abstract

We have developed a new procedure utilizing microhomologous recombination in yeast to generate targeting constructs for producing targeted mutations in mice. This procedure is rapid and efficient, and should be directly applicable to all mammalian genes. Moreover, only minimal information about the locus being targeted is required. The feasibility of this approach was demonstrated by producing another allele of the mouse Tg737 polycystic kidney gene.

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