Faculty Research 1990 - 1999

Title

Lymphoid and erythroid repopulation in B6 W-anemic mice: a new unirradiated recipient.

Document Type

Article

Publication Date

1991

Keywords

Animal, Bone-Marrow-Transplantation: ph, pa, Cell-Division: ph, Erythrocytes: ph, pa, Female, Hematopoiesis: ph, Hematopoietic-Stem-Cells: ph, pa, Lymphocytes: ph, pa, Male, Mice, Mice-Mutant-Strains, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Exp Hematol 1991 Jun; 19(5):374-7.

Grant

AG00594, DK25687, AG06232

Abstract

The W-anemic family of mouse mutants is an important model for studying repopulation in unirradiated recipients. This is the first study of blood lymphoid cell repopulation in adult W-anemic mutants given high doses of marrow cells, and it shows a wide difference in repopulation rates of circulating lymphoid and erythroid cells. This study also offers an improved model for marrow transplantation, using W alleles that are spontaneous mutations on the widely used inbred strain C57BL/6J (B6). Unirradiated B6-W41J/W41J or -W41J/W39J recipients of 2 x 10(6) B6 marrow cells are completely repopulated with donor erythrocytes after 3 months, whereas complete repopulation of lymphocytes requires a year. Surprisingly, the eventual degree of repopulation is independent of the severity of the mutation. The new mutants are not as anemic as the commonly used WBB6F1-W/Wv anemic mutants, they have a much higher ability to form macroscopic spleen colonies (spleen colony-forming units, CFU-S), and B6-W41J/W41J mice are fertile. Nevertheless, lymphoid and erythroid repopulation occur to a similar extent in B6-W41J/W41J or -W41J/W39J and in WBB6F1-W/Wv anemic mutants. Repopulation is more rapid in the latter, but host cells may be damaged by B6 reactions against the WB parent. Avoiding graft-versus-host reactions, hybrid resistance, and similar complications are important advantages in using donors and unirradiated recipients all on the B6 mouse genetic background. Additionally, congenic B6 mice provide a variety of genetic markers, allowing myeloid and lymphoid repopulation to be readily quantitated.

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