Faculty Research 1990 - 1999

Title

Tumor-derived products induce Il-1 a, Il-1 b, Tnf a, and Il-6 gene expression in murine macrophages: distinctions between tumor- and bacterial endotoxin-induced gene expression.

Document Type

Article

Publication Date

1991

Keywords

Cells-Cultured, Endotoxins, Escherichia-coli, Gene-Expression: de, Interleukin-1: ge, Interleukin-6: ge, Kinetics, Lipopolysaccharides, Macromolecular-Systems, Macrophages: de, im, ph, Mice, Mice-Inbred-C3H, Mice-Inbred-C57BL, RNA-Messenger: ge, Sarcoma-Experimental: pp, pa, SUPPORT-U-S-GOVT-P-H-S, Transcription-Genetic: de, Tumor-Necrosis-Factor: ge

JAX Location

2,133.

JAX Source

J Leukoc Biol 1991 May; 49(5):474-82.

Grant

R01CA27523

Abstract

The ability of progressing tumors to regulate host physiology is an important consideration in our understanding of tumor-host relationships. Previous data indicated that several lines of murine sarcoma cells produced one or more activities that were able to regulate both Il-1 a and Il-1 b gene transcription in macrophages (MO). We now describe an indepth analysis using Northern analysis and bioassays and show that two of these tumors produce one or more activities that when incubated with peritoneal MO result in the transcription of the Il-1a, Il-1 b, Tnf a, and Il-6 genes. Concordant with the Northern analyses was the finding that interleukin-1 (IL-1) and tumor necrosis factor (TNF) biological activities were detected in lysates of induced MO, fixed MO, and supernates of MO cultures. Induction of gene expression was shown to be distinct from that induced by bacterial endotoxin or lipopolysaccharide by a number of criteria. The data suggest that tumor cell products may play an important role in regulating several host physiological processes, particularly those involving Il-1a, Il-1 b, Tnf a, and Il-6 gene expression.

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