Faculty Research 1990 - 1999
Rescue of cyclin D1 deficiency by knockin cyclin E.
Document Type
Article
Publication Date
1999
Keywords
Cyclin-D1: ge, df, me, Cyclin-E: ge, me, Human, Mice, Mice-Inbred-C57BL, Phenotype, Receptors-Estrogen: me, Retina: me, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
First Page
767
Last Page
777
JAX Source
Cell 1999 Jun 11;97(6):767-77
Grant
535CA39826/CA/NCI
Abstract
D-type cyclins and cyclin E represent two very distinct classes of mammalian G1 cyclins. We have generated a mouse strain in which the coding sequences of the cyclin D1 gene (Ccnd1) have been deleted and replaced by those of human cyclin E (CCNE). In the tissues and cells of these mice, the expression pattern of human cyclin E faithfully reproduces that normally associated with mouse cyclin D1. The replacement of cyclin D1 with cyclin E rescues all phenotypic manifestations of cyclin D1 deficiency and restores normal development in cyclin D1-dependent tissues. Thus, cyclin E can functionally replace cyclin D1. Our analyses suggest that cyclin E is the major downstream target of cyclin D1.
Recommended Citation
Geng Y,
Whoriskey W,
Park MY,
Bronson RT,
Medema RH,
Li T,
Weinberg RA,
Sicinski P.
Rescue of cyclin D1 deficiency by knockin cyclin E. Cell 1999 Jun 11;97(6):767-77