Faculty Research 1990 - 1999

Title

FVB/N: an inbred mouse strain preferable for transgenic analyses.

Document Type

Article

Publication Date

3-15-1991

Keywords

Animal, Base-Sequence, Chimera, Comparative-Study, Female, Fertility, Genes-Viral, Genetic-Markers, Male, Mice, Mice-Inbred-Strains: ge, Mice-Transgenic, Molecular-Sequence-Data, Oligonucleotide-Probes, Polymerase-Chain-Reaction, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, SV40-Virus

JAX Source

Proc Natl Acad Sci USA 1991 Mar 15; 88(6):2065-9.

Grant

CA02662, CA39652, HD21970, +

Abstract

FVB/N mice offer a system suitable for most transgenic experiments and subsequent genetic analyses. The inbred FVB/N strain is characterized by vigorous reproductive performance and consistently large litters. Moreover, fertilized FVB/N eggs contain large and prominent pronuclei, which facilitate microinjection of DNA. The phenotype of large pronuclei in the zygote is a dominant trait associated with the FVB/N oocyte but not the FVB/N sperm. In experiments to generate transgenic mice, the same DNA constructs were injected into three different types of zygotes: FVB/N, C57BL/6J, and (C57BL/6J x SJL/J)F1. FVB/N zygotes survived well after injection, and transgenic animals were obtained with efficiencies similar to the F1 zygotes and much better than the C57BL/6J zygotes. Genetic markers of the FVB/N strain have been analyzed for 44 loci that cover 15 chromosomes and were compared with those of commonly used inbred strains. In addition to the albino FVB/N strain, pigmented congenic strains of FVB/N are being constructed. These features make the FVB/N strain advantageous to use for research with transgenic mice.