Faculty Research 1990 - 1999
FVB/N: an inbred mouse strain preferable for transgenic analyses.
Document Type
Article
Publication Date
3-15-1991
Keywords
Animal, Base-Sequence, Chimera, Comparative-Study, Female, Fertility, Genes-Viral, Genetic-Markers, Male, Mice, Mice-Inbred-Strains: ge, Mice-Transgenic, Molecular-Sequence-Data, Oligonucleotide-Probes, Polymerase-Chain-Reaction, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, SV40-Virus
First Page
2065
Last Page
2069
JAX Source
Proc Natl Acad Sci USA 1991 Mar 15; 88(6):2065-9.
Grant
CA02662, CA39652, HD21970, +
Abstract
FVB/N mice offer a system suitable for most transgenic experiments and subsequent genetic analyses. The inbred FVB/N strain is characterized by vigorous reproductive performance and consistently large litters. Moreover, fertilized FVB/N eggs contain large and prominent pronuclei, which facilitate microinjection of DNA. The phenotype of large pronuclei in the zygote is a dominant trait associated with the FVB/N oocyte but not the FVB/N sperm. In experiments to generate transgenic mice, the same DNA constructs were injected into three different types of zygotes: FVB/N, C57BL/6J, and (C57BL/6J x SJL/J)F1. FVB/N zygotes survived well after injection, and transgenic animals were obtained with efficiencies similar to the F1 zygotes and much better than the C57BL/6J zygotes. Genetic markers of the FVB/N strain have been analyzed for 44 loci that cover 15 chromosomes and were compared with those of commonly used inbred strains. In addition to the albino FVB/N strain, pigmented congenic strains of FVB/N are being constructed. These features make the FVB/N strain advantageous to use for research with transgenic mice.
Recommended Citation
Taketo M,
Schroeder AC,
Mobraaten LE,
Gunning KB,
Hanten G,
Fox RR,
Roderick TH,
Stewart CL,
Lilly F,
Hansen CT.
FVB/N: an inbred mouse strain preferable for transgenic analyses. Proc Natl Acad Sci USA 1991 Mar 15; 88(6):2065-9.