Faculty Research 1990 - 1999

Title

Mouse platelet-derived growth factor receptor alpha gene is deleted in W19H and patch mutations on chromosome 5.

Document Type

Article

Publication Date

1991

Keywords

Blotting-Southern, Chromosome-Deletion, Chromosome-Mapping, Cricetulus, DNA: ge, Female, Hamsters, Hybrid-Cells: cy, Linkage-(Genetics), Male, Mice, Mice-Inbred-C3H, Mice-Mutant-Strains: ge, Muridae, Mutation, Nucleic-Acid-Hybridization, Platelet-Derived-Growth-Factor: ph, Receptors-Cell-Surface: ge, Recombination-Genetic, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Proc Natl Acad Sci U S A 1991 Jun 1;88(11):4811-5

Grant

PO1AG06872/AG/NIA, HG00101/HG/NCHGR, DK27726/DK/NIDDK

Abstract

The mouse W19H mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor alpha gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W19H/ + heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W19H deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.

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