Faculty Research 1990 - 1999

Title

Modifications of the guanidine hydrochloride procedure for the extraction of RNA: isolation from a variety of tissues and adherent/nonadherent cell types.

Document Type

Article

Publication Date

1992

Keywords

Cell-Adhesion, Cells-Cultured, Cryopreservation, Genetic-Techniques, Guanidines, Male, Mice, Mice-Inbred-C57BL, RNA, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Biotechniques 1992 May;12(5):632-8

Grant

CA27523

Abstract

In a previous report dealing with the guanidine hydrochloride protocol for the extraction of RNA from mouse peritoneal macrophages, we identified a major source of RNA-degrading activity and showed that its removal early in the extraction procedure resulted in a more dependable method for the recovery of high-quality RNA. This report extends these findings and demonstrates the general applicability of the technique to a variety of fresh or frozen adherent cell types, cell suspensions and tissues, further highlighting stages at which degradation is most likely to occur and how to avoid a variety of pitfalls associated with the extraction procedure.

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