Mouse oocytes promote proliferation of granulosa cells from preantral and antral follicles in vitro.
Cell-Differentiation, Cell-Division, Cells-Cultured, Female, FSH, Graafian-Follicle: cy, su, Granulosa-Cells: cy, Mice, Mice-Inbred-C57BL, Oocytes: ph, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
Biol Reprod 1992 Jun;46(6):1196-204
Evidence is now emerging that the oocyte plays a role in the development and function of granulosa cells. This study focuses on the role of the oocyte in the proliferation of (1) undifferentiated granulosa cells from preantral follicles and (2) more differentiated mural granulosa cells and cumulus granulosa cells from antral follicles. Preantral follicles were isolated from 12-day-old mice, and mural granulosa cells and oocyte-cumulus complexes were obtained from gonadotropin-primed 22-day-old mice. Cell proliferation was quantified by autoradiographic determination of the 3H-thymidine labeling index. To determine the role of the oocyte in granulosa cell proliferation, oocyte-cumulus cell complexes and preantral follicles were oocytectomized (OOX), oocytectomy being a microsurgical procedure that removes the oocyte while retaining the three-dimensional structure of the complex or follicle. Mural granulosa cells as well as intact and OOX complexes and follicles were cultured with or without FSH in unconditioned medium or oocyte-conditioned medium (1 oocyte/microliter of medium). Preantral follicles were cultured for 4 days, after which 3H-thymidine was added to each group for a further 24 h. Mural granulosa cells were cultured as monolayers for an equilibration period of 24 h and then treated for a 48-h period, with 3H-thymidine added for the last 24 h. Oocyte-cumulus cell complexes were incubated for 4 h and then 3H-thymidine was added to each group for an additional 3-h period. FSH and/or oocyte-conditioned medium caused an increase in the labeling index of mural granulosa cells in monolayer culture; however, no differences were found among treatment groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Vanderhyden, B C.; Telfer, E E.; and Eppig, J J., " Mouse oocytes promote proliferation of granulosa cells from preantral and antral follicles in vitro." (1992). Faculty Research 1990 - 1999. 291.
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