Faculty Research 1990 - 1999

Title

Silver-enhanced radial immunodiffusion assay of plasma apolipoproteins.

Document Type

Article

Publication Date

1992

Keywords

Apolipoproteins, Immunodiffusion: mt, Mice, Sensitivity-and-Specificity, Silver, Stains-and-Staining, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

J Lipid Res 1992 Jul;33(7):1073-8

Grant

HL32087/HL/NHLBI

Abstract

Silver-staining of immunoprecipitates extends the sensitivity of the radial immunodiffusion assay by tenfold. This modification permits the quantification of apolipoproteins A-I, A-II, C, and E at levels of 0.2-1.0 mg/dl in plasma samples at a sensitivity threshold of 10 ng. The silver-enhanced radial immunodiffusion method is readily adapted from the standard method, simple and inexpensive to perform, and does not require costly instrumentation. These advantages make the modified RID assay an attractive alternative to other forms of immunoassay.

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