Faculty Research 1990 - 1999

Title

Localization of insulin-2 (Ins-2) and the obesity mutant tubby (tub) to distinct regions of mouse chromosome 7.

Document Type

Article

Publication Date

1992

Keywords

Base-Sequence, Chromosome-Mapping, Crosses-Genetic, Insulin: ge, Mice: ge, Mice-Inbred-C3H, Mice-Inbred-C57BL, Molecular-Sequence-Data, Muridae, Obesity: ge, Polymerase-Chain-Reaction, Restriction-Fragment-Length-Polymorphisms, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Genomics 1992 Sep;14(1):197-9

Grant

HG00101/HG/NCHGR, GM24872/GM/NIGMS, RR01183/RR/NCRR

Abstract

A DNA mapping panel derived from an interspecific backcross was used to position the mouse insulin-2 locus (Ins-2) on Chromosome 7, near H19 (0/114 recombinants) and Th (1/114 recombinants). Ins-2 is part of a human-mouse conserved linkage group that includes Th, H19, and Igf-2. Analysis of segregation in the F2 generation from the cross C57BL/6J-tub/tub x CAST/Ei demonstrated that Ins-2 and the obesity mutant tubby (tub) are distinct loci, thus eliminating Ins-2 as a candidate gene for tub. These results also refine the estimated genetic distance between tub and Hbb to 2.4 +/- 1.4 cM. The predicted location for a human homolog of tubby is HSA 11p15.

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