Faculty Research 1990 - 1999

Title

Induction of functional follicular dendritic cell development in severe combined immunodeficiency mice. Influence of B and T cells.

Document Type

Article

Publication Date

1993

Keywords

B-Lymphocytes: im, Bone-Marrow-Transplantation, Dendritic-Cells: im, pa, Lymph-Nodes: pa, Male, Mice, Mice-Inbred-BALB-C, Mice-Nude, Mice-SCID: im, Severe-Combined-Immunodeficiency: pa, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes: im

JAX Source

J Immunol 1993 Apr 1;150(7):2648-58

Grant

AG05374/AG/NIA, AI17142/AI/NIAID, AI30389/AI/NIAID

Abstract

Ag injected into immune mice immediately complexes with specific antibody. Immune complexes not phagocytosed by macrophages are transported by Ag transport cells to lymph node follicles for trapping by follicular dendritic cells (FDC). These FDC serve as a long term repository of unprocessed Ag that is believed to maintain both B cell memory and the secondary antibody response. Severe combined immunodeficiency mice lack functional B cells and T cells. Consequently, this mutation also appears to affect the ability to produce Ag-retaining FDC. to assess B and T cell requirements for FDC development and function, severe combined immunodeficiency mice were reconstituted with BM, or mature B and T cells. The development of a FDC reticulum, a three-dimensional network produced by the intertwining of FDC dendrites was assessed by Ag trapping on FDC using the histochemically detectable Ag horseradish peroxidase and quantitated by morphometry. The results showed that bone marrow transplants or B and T cells transferred together supply the required elements for the development of severe combined immunodeficiency FDC reticula that function in Ag trapping and the induction of the germinal center. In contrast, B cells or T cells injected separately induced a minimal development of FDC reticulum. The B and T cell requirements demonstrated here strongly indicate that B-T cell collaboration and the factors these cells produce are essential for FDC development.