Faculty Research 1990 - 1999

Title

Complex patterns of sequence variation and multiple 5' and 3' ends are found among transcripts of the erythroid ankyrin gene.

Document Type

Article

Publication Date

1993

Keywords

Animal, Ankyrins: ge, Base-Sequence, Cerebellum: ph, Cloning-Molecular, Comparative-Study, DNA: ge, DNA-Probes, Exons, Gene-Library, Mice, Mice-Inbred-C57BL, Molecular-Sequence-Data, Organ-Specificity, Polymerase-Chain-Reaction: mt, Restriction-Mapping, Reticulocytes: ph, Sequence-Homology-Amino-Acid, Spleen: ph, SUPPORT-U-S-GOVT-P-H-S, Transcription-Genetic, Variation-(Genetics)

JAX Source

J Biol Chem 1993 May 5;268(13):9533-40

Grant

HL29305/HL/NHLBI, DK27726/DK/NIDDK, HL15157/HL/NHLBI

Abstract

The structural protein ankyrin functions in red blood cells to link the spectrin-based membrane skeleton to the plasma membrane. Ankyrin proteins are now known to occur in most cell types, and two distinct ankyrin genes have been identified (erythroid (Ank-1) and brain (Ank-2)). We have characterized transcripts of the mouse erythroid ankyrin gene by cDNA cloning and DNA sequencing. Ank-1 transcripts of 7.5 and 9.0 kilobases are found in erythroid tissues, and a 9.0-kilobase transcript is found in cerebellum. RNA hybridization blot analysis of 13 additional mouse tissues has detected four novel Ank-1 transcripts (5.0, 3.5, 2.0, and 1.6 kilobases in size). Sequencing of Ank-1 cDNA clones isolated from mouse reticulocyte, spleen, and cerebellar libraries has identified (i) multiple 5' ends that indicate possible multiple promoters; (ii) alternative polyadenylation sites that probably account for the 7.5- and 9.0-kilobase size difference; (iii) a variety of small insertions and deletions that could produce transcripts (and ultimately proteins) of nearly identical size, but different functions; and (iv) clones with large deletions of coding sequence that account for the smaller transcripts seen in spleen, skeletal muscle, and heart.