Direct isolation of polymorphic markers linked to a trait by genetically directed representational difference analysis.

Authors

N A. Lisitsyn
J A. Segre
K Kusumi
N M. Lisitsyn
J H. Nadeau
W N. FrankelFollow
M H. Wigler
E S. Lander

Document Type

Article

Publication Date

1994

Keywords

Base-Sequence, Chromosome-Mapping, Cloning-Molecular, Crosses-Genetic, DNA-Primers: ge, Female, Genetic-Markers, Genetic-Techniques, Linkage-(Genetics), Male, Mice, Mice-Inbred-Strains: ge, Mice-Nude: ge, Molecular-Probe-Techniques, Molecular-Sequence-Data, Polymorphism-(Genetics), SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S

First Page

57

Last Page

63

JAX Source

Nat Genet 1994 Jan;6(1):57-63

Abstract

We describe a technique, genetically directed representational difference analysis (GDRDA), for specifically generating genetic markers linked to a trait of interest. GDRDA is applicable, in principle, to virtually any organism, because it requires neither prior knowledge of the chromosomal location of the gene controlling the trait nor the availability of a pre-existing genetic map. Based on a subtraction technique described recently called representational difference analysis, GDRDA uses the principles of transmission genetics to create appropriate Tester and Driver samples for subtraction. We demonstrate the usefulness of GDRDA by, for example, successfully targeting three polymorphisms to an interval of less than 1 cM of the mouse nude locus of chromosome 11.

Recommended Citation

Lisitsyn NA, Segre JA, Kusumi K, Lisitsyn NM, Nadeau JH, Frankel WN, Wigler MH, Lander ES. Direct isolation of polymorphic markers linked to a trait by genetically directed representational difference analysis. Nat Genet 1994 Jan;6(1):57-63