Faculty Research 1990 - 1999

Title

Effects of transplantation on the primitive immunohematopoietic stem cell.

Document Type

Article

Publication Date

1990

Keywords

Animal, B-Lymphocytes, Bone-Marrow-Transplantation, Erythrocytes, Hematopoietic-Stem-Cells, Male, Mice, Mice-Inbred-Strains, Support-U, S, -Gov't-P, H, S, Time-Factors

JAX Source

J Exp Med 1990 Aug 1; 172(2):431-7.

Grant

DK25687, AG00594, AG06232

Abstract

Transplantation has strong deleterious effects on the primitive immunohematopoietic stem cells (PSC) from which circulating lymphocytes and erythrocytes are descended. We studied these effects over 300-400 d, testing whether PSC numbers, repopulating abilities, or both, were reduced. Equivalent PSC numbers were estimated in recipients of mixtures of genetically different cells, using the binomial model with covariance. Percentages of lymphocyte and erythrocyte types were closely correlated, as were percentages of either type sampled at intervals of several months. This suggests that the same PSC produced lymphoid and myeloid cells, and that most circulating cells were descended from the same PSC over hundreds of days. Equivalent PSC concentrations were approximately 1/10(5) fresh marrow cells, and were about twofold lower using previously transplanted marrow. However, such marrow repopulated only one-seventh to one-eighth as well as fresh marrow. Apparently, transplantation not only reduces PSC concentrations, but also reduces the repopulating ability per PSC. This may result from excessive stimuli to differentiate that overbalance the stimuli for PSC to replenish themselves.