Faculty Research 1990 - 1999

Title

CD4+ T-lymphocytes are not required for murine resistance to the human filarial parasite, Brugia malayi.

Document Type

Article

Publication Date

1994

Keywords

Antibodies-Helminth, Antibodies-Monoclonal: im, Blotting-Western, Brugia-malayi: im, Enzyme-Linked-Immunosorbent-Assay, Female, Filariasis: im, Immunoglobulin-Isotypes: bi, Leukocyte-Count, Lymphocyte-Subsets: im, Male, Mice, Mice-Inbred-BALB-C, Mice-SCID, Specific-Pathogen-Free-Organisms, Spleen: cy, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, T4-Lymphocytes: im

JAX Source

Exp Parasitol 1994 Jun;78(4):352-60

Grant

AI27773/AI/NIAID, AI30046/AI/NIAID, AI30389/AI/NIAID

Abstract

Immunocompetent mice are nonpermissive for the development and maturation of the human filarial parasite, Brugia malayi. We and others have shown that the absence of T-lymphocytes, alone or in combination with B-lymphocytes, renders mice permissive to infection. In a previous study, we showed that mice lacking CD8+ T-lymphocytes are also completely nonpermissive for B. malayi, indicating that CD8+ T-lymphocytes are not an obligate requirement for resistance. In the present study, we have examined the role of CD4+ T-lymphocytes in resistance to filarial infection using two experimental systems. In the first, we used an anti-CD4 monoclonal antibody to deplete CD4+ T-cells in vivo in immunocompetent BALB/c mice. In the second system, we used mutant mice in which the gene encoding the CD4 antigen had been disrupted by homologous recombination, resulting in a lack of CD4+ T-cells. Challenge of either the anti-CD4 antibody depleted BALB/c mice or CD4 knockout mice with B. malayi infective-stage larvae demonstrated that mice lacking CD4+ T-lymphocytes were resistant to infection. These data indicate that CD4+ T-cells are not an obligate requirement for murine resistance to B. malayi.

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