Faculty Research 1990 - 1999

Title

Gene regulation during neuronal and non-neuronal differentiation of NTERA2 human teratocarcinoma-derived stem cells.

Document Type

Article

Publication Date

1994

Keywords

Base-Sequence, Cell-Differentiation: de, ge, Cell-Separation, Flow-Cytometry, Gene-Expression-Regulation-Neoplastic: ph, Genomic-Library, Human, Molecular-Sequence-Data, SUPPORT-U-S-GOVT-P-H-S, Teratocarcinoma: ge, pa, Tretinoin, Tumor-Cells-Cultured, Tumor-Stem-Cells: cy

JAX Source

Brain Res Mol Brain Res 1994 Aug;25(1-2):157-62

Grant

CA29894/CA/NCI, CA37725/CA/NCI, CA34196/CA/NCI

Abstract

We constructed cDNA libraries from a clonal human teratocarcinoma-derived cell line and two retinoic acid-induced derivatives: a homogeneous population of neurons and a FACS-isolated, non-neuronal population. These libraries are large and representative of the cells from which they were derived, as determined by colony hybridization. PCR analysis indicates that the transcripts encoding P- and E-cadherin are down-regulated whereas the the prion protein (PrP) transcript is up-regulated in neurons. These cells offer a promising system for investigations of human prion infection and the cDNA libraries provide a source of neuron-specific genes.

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