Faculty Research 1990 - 1999

Title

A segment of the MHC class II beta chain plays a critical role in targeting class II molecules to the endocytic pathway.

Document Type

Article

Publication Date

1994

Keywords

Antigen-Presenting-Cells, Base-Sequence, DNA-Primers, Endocytosis, Endosomes, Fibroblasts, Fluorescent-Antibody-Technique, Histocompatibility-Antigens-Class-II, L-Cells-(Cell-Line), Mice, Molecular-Sequence-Data, Transfection

JAX Source

Int Immunol 1994 Jul; 6(7):973-82.

Abstract

The ability of MHC class II molecules to sort into the endocytic pathway has generally been attributed to the invariant chain glycoprotein. In this paper, we present evidence suggesting that lumenal sequences in the MHC" class II molecule itself control the post-Golgi entry of class II into endosomes. Single amino acid changes have been introduced into a highly conserved region of the class II beta chain (amino acids 80-83). Mutant class II beta chain genes and wild-type alpha chain genes have been transfected into cells that lack both class II and invariant chain expression. Immunofluorescent staining of transfected cells indicates that single amino acid changes in this region of beta can positively or negatively modulate expression of class II in endocytic vesicles independently of invariant chain. Mutation at residue 80 leads to prominent localization in vesicular structures typical of late endocytic compartments, while a change at position 82 leads to arrest in the Golgi." These data argue in favor of the possibility that MHC class II molecules bear a sorting signal that allows access to MHC class II molecules into the endocytic pathway of antigen presenting cells.

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