Faculty Research 1990 - 1999

Title

Survival of human prostate carcinoma, benign hyperplastic prostate tissues, and IL-2-activated lymphocytes in scid mice.

Document Type

Article

Publication Date

1995

Keywords

Animal, Cell-Survival, Disease-Models-Animal, Flow-Cytometry, Human, Interleukin-2, Lymphocyte-Subsets, Lymphocytes-Tumor-Infiltrating: pa, Male, Mice, Mice-SCID, Neoplasm-Transplantation, Neoplasms-Hormone-Dependent: bl, pa, Prostate-Specific-Antigen, Prostatic-Hypertrophy: bl, pa, Prostatic-Neoplasms: bl, pa, Testosterone, Tumor-Cells-Cultured

JAX Source

Prostate 1995 Jul;27(1):32-41

Abstract

Mice, homozygous for the mutation severe combined immunodeficiency (scid) and also segregating for the mutation hypogonadal (hpg), were tested for their potential use as an in vivo model system for studying the growth of human prostate cancer and benign hyperplastic prostate tissue grafts. Fresh human prostate cancer or benign hyperplastic prostate tissue was implanted subcutaneously into androgen-replete C.B. 17 scid/scid males, and into androgen-deficient hpg/hpg scid/scid or androgen-replete +/? scid scid males. The tissue grafts grew in both androgen-replete and androgen-deficient host mice. When dihydrotestosterone (DHT) was administered at tissue grafting, both the incidence and size of the tissue grafts increased. Histology of tissue from tumors in the androgen-deficient hpg/hpg scid/scid host showed either undifferentiated tumors or adenocarcinomas with few glandular structures. These data suggest the androgen deficient environment selected for growth of androgen-independent tumor tissue. Finally, when interleukin-2 (IL-2)-activated tumor-infiltrating lymphocytes were injected into scid/scid hosts, the cells were found to survive and could be identified in the spleen of the recipient mice. These results indicate that growth of human prostate tissues and IL-2-activated lymphocytes in scid/scid mice is a viable model system for in vivo studies of prostatic disease.

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