Faculty Research 1990 - 1999

Title

Characterization of a new antigen expressed by B and myeloid lineage cells identified by the monoclonal antibody LIP-6.

Document Type

Article

Publication Date

1995

Keywords

Antibodies-Monoclonal: bi, Antigen-Antibody-Reactions, Antigens-Differentiation: im, B-Lymphocytes: im, Bone-Marrow: im, Cell-Line, Hematopoietic-Stem-Cells: im, Hybridomas: me, Mice, Mice-Inbred-BALB-C, Mice-Inbred-C3H, Mice-Inbred-C57BL, Peritoneal-Cavity: cy, Plasma-Cells: im, Rats, Rats-Inbred-Lew, Spleen: im, Tissue-Distribution: im

JAX Source

Cell Immunol 1995 Nov;166(1):131-40

Abstract

The LIP-6 MAb was produced against the undifferentiated cell line bh2-1 and recognizes an antigen expressed on all pre-B and B cell lines tested and some myeloid lineage lines. FACS analysis of normal tissues showed that LIP-6 is expressed on B lineage cells at all stages of differentiation, from bone marrow pre-B to plasma cells. T cells and thymocytes are LIP-6-, and splenic CD11b+ cells are heterogeneous for LIP-6 expression. The LIP-6 MAb was shown to precipitate a major 75-kDa and a minor 85-kDa protein under reducing conditions and a large protein of > 240 kDa under nonreducing conditions. Removal of N-linked sugars from the reduced lysates resulted in a single 65-kDa protein, suggesting that there is differential glycosylation of a single 65-kDa protein that forms disulfide-linked multimers. Finally, the LIP-6 antigen was shown not to be linked to the cell surface via a GPI linkage.

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