Faculty Research 1990 - 1999

Title

Efficient isolation of novel mouse genes differentially expressed in early postimplantation embryos.

Document Type

Article

Publication Date

1995

Keywords

Base-Sequence, DNA, Female, Gene-Expression-Regulation-Developmental, In-Situ-Hybridization, Male, Mice, Mice-Inbred-BALB-C, Mice-Inbred-C57BL, Molecular-Sequence-Data, Pilot-Projects, Postimplantation-Phase: ge, Pregnancy, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Genomics 1995 Aug 10;28(3):436-41

Grant

CA34196/CA/NCI

Abstract

Most genes with regulatory functions in embryogenesis are expressed in highly specific patterns, suggesting that expression patterns can serve as criteria to define potential candidates for developmentally relevant genes. To isolate such genes, we selected and partially sequenced 80 cDNA clones from a 10.5-day mouse embryo library. Forty-one clones that represented novel mouse genes were analyzed for expression in embryos of the same stage by whole-mount in situ hybridization. A high proportion (24%) of these genes, including a homologue of the Drosophila Delta gene, were expressed in specific spatially restricted patterns, suggesting that selection based on expression patterns is a useful strategy to isolate novel genes that may play pivotal roles in mammalian development.

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