Faculty Research 1990 - 1999
Chromosomal locations of major tRNA gene clusters of Xenopus laevis.
Document Type
Article
Publication Date
1995
Keywords
Chromosome-Mapping, Genes-Reiterated, In-Situ-Hybridization, Repetitive-Sequences-Nucleic-Acid, Restriction-Mapping, RNA-Transfer: ge, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Xenopus-laevis: ge
First Page
68
Last Page
74
JAX Source
Chromosoma 1995 Oct;104(1):68-74
Abstract
In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly repeated unit. There are 150 copies of the unit at a single locus near the long arm telomere of one of the acrocentric chromosomes in the 14-17 group. Two additional classes of tRNA gene-containing repeats have been isolated (defined by clones p3.1 and p3.2) that have structures related to that of the 3.18 kb unit. Using in situ hybridization at the electron microscopic level, the p3.2 repeats are found clustered at a single locus in the subtelomeric region on one of the submetacentric chromosomes, whereas the p3.1 repeats are clustered at a locus indistinguishable from that containing the 3.18 kb repeats. This suggests that these tDNA tandem repeats can diverge in sequence from each other without being at distantly separated loci.
Recommended Citation
Narayanswami S,
Doering JL,
Fokta FJ,
Rosenthal DS,
Nguyen TN,
Hamkalo BA.
Chromosomal locations of major tRNA gene clusters of Xenopus laevis. Chromosoma 1995 Oct;104(1):68-74