Faculty Research 1990 - 1999

Title

Mediation of interleukin-11-dependent biological responses by a soluble form of the interleukin-11 receptor.

Document Type

Article

Publication Date

1996

Keywords

Antigens-CD: me, B-Lymphocytes, Cell-Division: de, Cell-Line, Electrophoresis-Polyacrylamide-Gel, Genetic-Vectors, Human, Interleukin-11: me, Kinetics, Membrane-Glycoproteins: me, Mice, Polymerase-Chain-Reaction, Receptors-Interleukin: de, ph, Recombinant-Proteins: de, me, Signal-Transduction, SUPPORT-NON-U-S-GOVT, Transfection

JAX Source

Biochem J 1996 Sep 1;318 ( Pt 2):489-95

Abstract

Interleukin-11 (IL-11) is a polyfunctional cytokine whose biological actions require a specific IL-11 receptor (IL-11R) and the transmembrane transducer gp130. Here we report the production of a soluble form of the murine IL-11R and demonstrate that it interacts with IL-11 ligand with high affinity. The affinity of IL-11 alone for gp130 is below the level of detection, but a complex of IL-11 and soluble IL-11R interacts with gp130 with high affinity. The addition of soluble IL-11R potentiates the effects of exogenous IL-11 in cells that are normally responsive to IL-11. A biological response to IL-11 can be reconstituted in BAF cells transfected with gp130 by addition of IL-11 and soluble IL-11R. These findings show that the cytoplasmic domain of the IL-11R is not required for the biological effects of IL-11 and that a complex of IL-11 and IL-11R mediates signalling by association with gp130.