Faculty Research 1990 - 1999

Title

Two differentially expressed interleukin-11 receptor genes in the mouse genome.

Document Type

Article

Publication Date

1996

Keywords

Animal, Base-Sequence, Cloning-Molecular, Comparative-Study, DNA-Primers, Gene-Expression, Genome, Interleukin-11: me, Mice: ge, Molecular-Sequence-Data, Polymerase-Chain-Reaction, Receptors-Interleukin: bi, ge, me, Recombinant-Proteins: bi, RNA-Messenger: bi, Sequence-Homology-Amino-Acid, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Biochem J 1996 Dec 1;320 ( Pt 2):359-63

Abstract

Interleukin-11 (IL-11) is a multifunctional cytokine involved in the regulation of cell proliferation and differentiation in a variety of cell types and tissues in vitro and in vivo. The effects of IL-11 were shown to be mediated by the IL-11 receptor (hereafter referred to as IL-11 R alpha), which is a ligand-binding subunit and provides ligand specificity in a functional multimeric signal-transduction complex with gp130. Here we show that the mouse genome contains a second gene encoding an IL-11-binding protein, referred to as IL-11R beta. The structure of the IL-11R beta gene is highly similar to that of IL-11R alpha, and IL-11R beta exhibits 99% sequence identity with IL-11R alpha at the amino acid level. IL-11R beta is co-expressed with IL-11R alpha, albeit at lower levels, in embryos and in various adult tissues. IL-11R beta transcripts are abundant in testis, and, in contrast with IL-11R alpha, absent from skeletal muscle. IL-11R beta expressed in vitro binds IL-11 with high affinity, suggesting that the mouse genome contains a second functional IL-11R.