Faculty Research 1990 - 1999

Title

Distribution and specific identification of papillomavirus major capsid protein epitopes by immunocytochemistry and epitope scanning of synthetic peptides.

Document Type

Article

Publication Date

1990

Keywords

Antibodies-Monoclonal: im, Antigenic-Determinants: an, Antigens-Viral: an, Capsid: im, Fibroma: mi, Immune-Sera: im, Immunohistochemistry, Papilloma: mi, Papillomaviruses: im, Reagent-Kits-Diagnostic, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Tumor-Virus-Infections: mi

JAX Source

J Infect Dis 1990 Dec; 162(6):1263-9.

Grant

CA50182

Abstract

Monoclonal (MAbs) and polyclonal antibodies were produced against the major capsid protein of detergent-disrupted, purified bovine papillomavirus type 1 (BPV-1). The precise locations of the corresponding epitopes were identified by the reactivity of MAbs and selected polyclonal antibodies with synthetic, overlapping, hexameric peptides corresponding with 95% of the BPV-1 major capsid protein. The topography of these epitopes was determined by reactivity of antibodies with intact (conformational and nonconformational surface epitopes) and disrupted (external or internal nonconformational epitopes) BPV-1 virions. The distribution of epitopes in various papillomaviruses of 13 different species was determined by reactivity of the MAbs and polyclonal sera with productively infected, formalin-fixed papillomas, fibropapillomas, and fibromas. Epitope scanning, using MAbs and polyclonal antisera, resulted in the precise location of BPV-1 hexameric epitopes that could be correlated with their topography on the capsid and distribution in papillomatous lesions of various species.

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