Faculty Research 1990 - 1999

Title

Glycosylphosphatidylinositol-anchored H-2Db molecules are defective in antigen processing and presentation to cytotoxic T lymphocytes.

Document Type

Article

Publication Date

1996

Keywords

Antigen-Presentation, Cells-Cultured, Cytotoxicity-Immunologic, Glycosylphosphatidylinositols: an, ph, H-2-Antigens: an, ph, Male, Mice, Mice-Inbred-CBA, Mice-Inbred-C3H, Mice-Inbred-C57BL, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes-Cytotoxic: im, Transfection

JAX Source

Eur J Immunol 1996 Sep;26(9):2215-24

Grant

GM46467/GM/NIGMS, DK38452/DK/NIDDK, AI24562/AI/NIAID

Abstract

Glycosylphosphatidylinositol-anchored (GPI)-Db molecules are defective in mediating cytotoxic T lymphocytes (CTL) lysis of transfected lymphoma cells, compared to their transmembrane (TM) counterpart. This defect is manifest when antigenic peptide must be processed and presented through the endogenous pathway. These same transfectants can be lysed by allospecific CTL, or by antigen-specific Db-restricted CTL when pulsed with appropriate exogenous synthetic peptide, demonstrating that they can bind and present peptide for CTL-mediated lympholysis. The defect apparently results from differences between GPI-Db and TM-Db assembly and transport, or from differences in membrane topology that affect CD8+ CTL recognition of major histocompatibility complex/peptide complex.