Faculty Research 1990 - 1999

Title

Physical and genetic mapping of novel microsatellite polymorphisms on human chromosome 19.

Document Type

Article

Publication Date

1996

Keywords

DNA-Satellite: ge, Genetic-Markers, Haplotypes, Human, In-Situ-Hybridization-Fluorescence, Linkage-(Genetics), Polymorphism-(Genetics), Recombination-Genetic, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Genomics 1996 Oct 1;37(1):125-30

Grant

HL48719/HL/NHLBI

Abstract

We describe here the identification of 11 novel microsatellite polymorphisms on human chromosome 19. These dinucleotide repeat polymorphisms were detected in chromosome 19-specific cosmids that were physically mapped by fluorescence in situ hybridization. For each repeat, flanking oligonucleotide primers were synthesized and the polymerase chain reaction assay was performed on a panel of 100 unrelated individuals to determine the heterozygosity and allele frequencies. To characterize these markers further, genetic and radiation hybrid maps were constructed. These microsatellite polymorphisms will be valuable in further linkage analysis of inherited diseases on chromosome 19p.