Faculty Research 1990 - 1999
Intron/exon structure confirms that mouse Zfy1 and Zfy2 are members of the ZFY gene family.
Document Type
Article
Publication Date
1997
Keywords
Base-Sequence, Comparative-Study, DNA: ge, DNA-Binding-Proteins: ge, Evolution-Molecular, Exons, Human, Introns, Mice, Molecular-Sequence-Data, Multigene-Family, Species-Specificity, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Y-Chromosome: ge, Zinc-Fingers: ge
First Page
123
Last Page
127
JAX Source
Genomics 1997 Apr 1;41(1):123-7
Grant
CA34196/CA/NCI, HD22532/HD/NICHD
Abstract
Zfy1 and Zfy2 are homologous zinc finger genes on the mouse Y Chromosome. To ask whether these genes are properly classified as members of the ZFY family, we have characterized and compared their genomic organization to that of mouse Zfx, human ZFX, and human ZFY. We show that Zfy1 has 11 exons distributed across at least 56 kb, and Zfy2 has a minimum of 9 exons distributed across at least 52 kb. The Zfy2 locus contains regions similar in size and sequence to all 11 exons of Zfy1, plus an additional 5' UTR exon. All splice sites conform to the GT-AG rule. There are two instances of additional AG dinucleotides immediately 5' of 3' splice sites. Zfy1 and Zfy2 are homologous to other ZFY family members within the coding region, but the untranslated regions show no sequence similarity. Within the coding region, there is conservation of exon length and splice sites, with each splice preceding the second nucleotide of a codon. We conclude that Zfy1 and Zfy2 are indeed members of the ZFY family, which has evolved from a single common ancestral gene.
Recommended Citation
Mahaffey CL,
Bayleran JK,
Yeh GY,
Lee TC,
Page DC,
Simpson EM.
Intron/exon structure confirms that mouse Zfy1 and Zfy2 are members of the ZFY gene family. Genomics 1997 Apr 1;41(1):123-7