Faculty Research 1990 - 1999

Title

Cisplatin increases meiotic crossing-over in mice.

Document Type

Article

Publication Date

1997

Keywords

Antineoplastic-Agents, Chromosome-Mapping: mt, Cisplatin, Crossing-Over-(Genetics): de, Meiosis: ge, Mice, Mice-Inbred-DBA, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Proc Natl Acad Sci U S A 1997 Aug 5;94(16):8681-5

Grant

GM45415/GM/NIGMS

Abstract

Genetic mapping of traits and mutations in mammals is dependent upon linkage analysis. The resolution achieved by this method is related to the number of offspring that can be scored and position of crossovers near a gene. Higher precision mapping is obtained by expanding the collection of progeny from an appropriate cross, which in turn increases the number of potentially informative recombinants. A more efficient approach would be to increase the frequency of recombination, rather than the number of progeny. The anticancer drug cisplatin, which causes DNA strand breakage and is highly recombinogenic in some model organisms, was tested for its ability to induce germ-line recombination in mice. Males were exposed to cisplatin and mated at various times thereafter to monitor the number of crossovers inherited by offspring. We observed a striking increase on all three chromosomes examined and established a regimen that nearly doubled crossover frequency. The timing of the response indicated that the crossovers were induced at the early pachytene stage of meiosis I. The ability to increase recombination should facilitate genetic mapping and positional cloning in mice.

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