Murine hematopoietic stem cell characterization and its regulation in BM transplantation.
Document Type
Article
Publication Date
2000
Keywords
Antigens-CD34, Antigens-Differentiation, Bone-Marrow-Transplantation, Colony-Forming-Units-Assay, Hematopoiesis, Hematopoietic-Stem-Cells, Immunophenotyping, Male, Mice, Mice-Inbred-C57BL, NAD+-Nucleosidase, SUPPORT-NON-U-S-GOVT, Time-Factors
First Page
3016
Last Page
3022
JAX Source
Blood 2000 Nov; 96(9):3016-22.
Abstract
Using 5-color fluorescence-activated cell sorting, we isolated a subset of murine pluripotent hematopoietic stem cells (PHSC) with the phenotype Lin(-) Sca(+) kit(+) CD38(+) CD34(-) that appears to fulfill the criteria for most primitive PHSC. In the presence of whole bone marrow (BM) competitor cells, these cells produced reconstitution in lethally irradiated primary, secondary, and tertiary murine transplant recipients over the long term. However, these cells alone could not produce reconstitution in lethally irradiated recipients. Rapid proliferation of these cells after BM transplantation required the assistance of another BM cell subset, which has the phenotype Lin(-) Sca(+) kit(+) CD38(-) CD34(+).
Recommended Citation
Zhao Y,
Lin Y,
Zhan Y,
Yang G,
Louie J,
Harrison DE,
Anderson WF.
Murine hematopoietic stem cell characterization and its regulation in BM transplantation. Blood 2000 Nov; 96(9):3016-22.