Title

In vitro fertilization with cryopreserved inbred mouse sperm.

Document Type

Article

Publication Date

2000

Keywords

Blastocyst, Cryopreservation, Embryo-Transfer, Female, Fertilization-in-Vitro, Male, Mice, Mice-Inbred-Strains, Ovum, Pregnancy, Semen-Preservation, Species-Specificity, Sperm-Motility, Spermatozoa, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Biol Reprod 2000 Dec; 63(6):1774-80.

Grant

RR01262/RR/NCRR, RR09781/RR/NCRR, RR15012/RR/NCRR

Abstract

Sperm from C57BL/6J, DBA/2J, BALB/cJ, 129S3/SvImJ, and FVB/NJ inbred mice were cryopreserved in 3% skim milk/18% raffinose cryoprotectant solution. The post-thaw sperm from all strains were evaluated for their viability and fertility by comparing them against B6D2F1 sperm used as a control. The protocol used for freezing mouse sperm was effective in different strains, because the motility was decreased by 50% after cryopreservation similar to other mammalian sperm. However, the progressive motility and the fertility of each inbred strain were affected differently. The C57BL/6J, BALB/cJ, and 129S3/SvImJ strains were the most affected; their fertility (two-cell cleavage) decreased from 70%, 34%, and 84% when using freshly collected sperm to 6%, 12%, and 6% when using frozen/thawed sperm, respectively. Live newborns derived from frozen/thawed sperm were obtained from all strains in the study. These results corroborate the genetic variation among strains with regard to fertility and susceptibility to cryopreservation.

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