Imaging axonal transport of mitochondria in vivo.
Document Type
Article
Publication Date
2007
Keywords
Axonal-Transport, Axons, Luminescent-Proteins, Mice, Mitochondria, Neurons, Spectrometry-Fluorescence
First Page
559
Last Page
561
JAX Location
see Reprint Collection (a pdf is available)
JAX Source
Nat Methods 2007 Jul; 4(7):559-61.
Abstract
Neuronal mitochondria regulate synaptic physiology and cellular survival, and disruption of their function or transport causes neurological disease. We present a fluorescence method to selectively image mitochondrial dynamics in the mouse nervous system, in both live mice and acute explants. We show that axon damage and recovery lead to early and sustained changes in anterograde and retrograde transport. In vivo imaging of mitochondria will be a useful tool to analyze this essential organelle.
Recommended Citation
Misgeld T,
Kerschensteiner M,
Bareyre FM,
Burgess RW,
Lichtman JW.
Imaging axonal transport of mitochondria in vivo. Nat Methods 2007 Jul; 4(7):559-61.