Improved insulin-sensitivity in mice heterozygous for PPAR-gamma deficiency.
DNA-Binding-Proteins, Glucose, Heterozygote, Homozygote, Insulin, Insulin-Resistance, Mice, Mutation, Receptors-Cytoplasmic-and-Nuclear, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Transcription-Factors
J Clin Invest 2000 Feb; 105(3):287-92.
The thiazolidinedione class of insulin-sensitizing, antidiabetic drugs interacts with peroxisome proliferator-activated receptor gamma (PPAR-gamma). To gain insight into the role of this nuclear receptor in insulin resistance and diabetes, we conducted metabolic studies in the PPAR-gamma gene knockout mouse model. Because homozygous PPAR-gamma-null mice die in development, we studied glucose metabolism in mice heterozygous for the mutation (PPAR-gamma(+/-) mice). We identified no statistically significant differences in body weight, basal glucose, insulin, or FFA levels between the wild-type (WT) and PPAR-gamma(+/-) groups. Nor was there a difference in glucose excursion between the groups of mice during oral glucose tolerance test, but insulin concentrations of the WT group were greater than those of the PPAR-gamma(+/-) group, and insulin-induced increase in glucose disposal rate was significantly increased in PPAR-gamma(+/-) mice. Likewise, the insulin-induced suppression of hepatic glucose production was significantly greater in the PPAR-gamma(+/-) mice than in the WT mice. Taken together, these results indicate that - counterintuitively - although pharmacological activation of PPAR-gamma improves insulin sensitivity, a similar effect is obtained by genetically reducing the expression levels of the receptor.
Miles, P D.; Barak, Y; He, W; Evans, R M.; and Olefsky, J M., " Improved insulin-sensitivity in mice heterozygous for PPAR-gamma deficiency." (2000). Faculty Research 2000 - 2009. 162.