Title

Role of modulator recognition factor 2 in adipogenesis and leptin expression in 3T3-L1 cells.

Document Type

Article

Publication Date

2008

Keywords

Adipogenesis, Animals, Cell-Differentiation, DNA-Binding-Proteins, Gene-Expression-Regulation-Developmental, Leptin, Mice, Transcription-Factors

JAX Source

Biochem Biophys Res Commun 2008 Feb; 366(2):551-5.

Abstract

The complex network of adipogenic transcription factors regulates adipocyte differentiation, obesity, and insulin resistance. Modulator recognition factor (Mrf) 2 knockout mice exhibit defects in fat accumulation and are protected from diet-induced obesity, suggesting that Mrf2 deficiency affects adipogenesis. Here, we report that the gene expressions of the 2 isoforms of the transcription factors Mrf2, Mrf2alpha, and Mrf2beta, were induced upon adipogenesis in 3T3-L1 cells. Mrf2 mRNA expression was sensitive to stimulation by insulin, dexamethasone, and TNF-alpha in 3T3-L1 preadipocytes and differentiated adipocytes. Down-regulation of Mrf2alpha and Mrf2beta gene expressions induced by small interfering RNAs increased the mRNA expression of leptin. These results indicate that Mrf2 can be a potential regulator of adipocyte differentiation and a potential repressor of leptin.