Human acute leukemia cells injected in NOD/LtSz-scid/IL-2Rgamma null mice generate a faster and more efficient disease compared to other NOD/scid-related strains.
Document Type
Article
Publication Date
2008
Keywords
Cell-Line-Tumor, Disease-Models-Animal, Humans, Immunohistochemistry, Interleukin-Receptor-Common-gamma-Subunit, Leukemia-Myeloid-Acute, Mice-Inbred-NOD, Mice-SCID, Neoplasm-Transplantation, Polymerase-Chain-Reaction, Precursor-Cell-Lymphoblastic-Leukemia-Lymphoma, Transplantation-Heterologous
First Page
2222
Last Page
2227
JAX Source
Int J Cancer 2008 Nov; 123(9):2222-7.
Abstract
Transplantation of human acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) primary cells and cell lines in different strains of immunodeficient mice has led to preclinical models extensively used to investigate acute leukemia stem cells, biology and drug sensitivity. We studied the engraftment kinetics of AML and ALL cell lines and primary cells in 3 strains of NOD.CB17-Prkdc(scid) (NOD/scid, NS)-related mice (NOD.Cg-Prkdc(scid)B2m(tm1Unc)/J, abbreviated NOD/scid/beta2 null, NSB; and NOD.Cg-Prkdc(scid)Il2rg(tm1Wjll)/SzJ, abbreviated NOD/scid/IL-2Rgamma null, NSG). The engraftment of human malignant cells was investigated by means of clinicopathological criteria, flow cytometry, PCR and immunohistochemistry. In NSG mice, we observed a significantly faster development of leukemia-related symptoms and a higher percentage of leukemia cells in the blood, in the marrow and in the spleen. The leukemia-related angiogenic switch (measured as the number of circulating endothelial cells and progenitors) was faster in NSG compared to NS and NSB mice. These models will be instrumental to studies on leukemia-initiating stem cells, leukemia biology, preclinical treatment studies, and to obtain patient-specific preclinical models to design and investigate patient-tailored therapies.
Recommended Citation
Agliano A,
Martin PI,
Mancuso P,
Marighetti P,
Rabascio C,
Pruneri G,
Shultz LD,
Bertolini F.
Human acute leukemia cells injected in NOD/LtSz-scid/IL-2Rgamma null mice generate a faster and more efficient disease compared to other NOD/scid-related strains. Int J Cancer 2008 Nov; 123(9):2222-7.