Title

mTORC1 promotes survival through translational control of Mcl-1.

Document Type

Article

Publication Date

2008

Keywords

Gene-Expression-Regulation-Neoplastic, Immunoblotting, Immunoprecipitation, Lymphoma, Mice, Proto-Oncogene-Proteins-c-bcl-2, Reverse-Transcriptase-Polymerase-Chain-Reaction, Signal-Transduction, Sirolimus, Transcription-Factors, Tumor-Suppressor-Proteins

JAX Source

Proc Natl Acad Sci U S A 2008 Aug; 105(31):10853-8.

Abstract

Activation of the phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway is a frequent occurrence in human cancers and a major promoter of chemotherapeutic resistance. Inhibition of one downstream target in this pathway, mTORC1, has shown potential to improve chemosensitivity. However, the mechanisms and genetic modifications that confer sensitivity to mTORC1 inhibitors remain unclear. Here, we demonstrate that loss of TSC2 in the E mu-myc murine lymphoma model leads to mTORC1 activation and accelerated oncogenesis caused by a defective apoptotic program despite compromised AKT phosphorylation. Tumors from Tsc2(+/-)E mu-Myc mice underwent rapid apoptosis upon blockade of mTORC1 by rapamycin. We identified myeloid cell leukemia sequence 1 (Mcl-1), a bcl-2 like family member, as a translationally regulated genetic determinant of mTORC1-dependent survival. Our results indicate that the extent by which rapamycin can modulate expression of Mcl-1 is an important feature of the rapamycin response.