Many ribosomal protein mutations are associated with growth impairment and tumor predisposition in zebrafish.
Document Type
Article
Publication Date
2009
Keywords
Chimera, Disease-Susceptibility, Gene-Knockdown-Techniques, Mutation, Nerve-Sheath-Neoplasms, Ribosomal-Proteins, Zebrafish, Zebrafish-Proteins
First Page
76
Last Page
85
JAX Source
Dev Dyn 2009 Jan; 238(1):76-85.
Abstract
We have characterized 28 zebrafish lines with heterozygous mutations in ribosomal protein (rp) genes, and found that 17 of these are prone to develop zebrafish malignant peripheral nerve sheath tumors (zMPNST). Heterozygotes from the vast majority of tumor-prone rp lines were found to be growth-impaired, though not all growth-impaired rp lines were tumor-prone. Significantly, however, the rp lines with the greatest incidence of zMPNSTs all displayed a growth impairment. Furthermore, heterozygous cells from one tumor-prone rp line were out-competed by wild-type cells in chimeric embryos. The growth impairment resulting from heterozygosity for many rp genes suggests that a global defect in protein translation exists in these lines, raising the possibility that a translation defect that precedes tumor development is predictive of tumorigenesis.
Recommended Citation
Lai K,
Amsterdam A,
Farrington S,
Bronson RT,
Hopkins N,
Lees JA.
Many ribosomal protein mutations are associated with growth impairment and tumor predisposition in zebrafish. Dev Dyn 2009 Jan; 238(1):76-85.