Title

Encephalitogenic T cells that stably express both T-bet and ROR gamma t consistently produce IFNgamma but have a spectrum of IL-17 profiles.

Document Type

Article

Publication Date

2009

Keywords

Clone-Cells, Encephalomyelitis-Autoimmune-Experimental, Gene-Expression-Profiling, Immunophenotyping, Interferon-gamma, Interleukin-17, Mice, Mice-Inbred-BALB-C, Mice-Transgenic, Myelin-Basic-Proteins, Receptors-Retinoic-Acid, Receptors-Thyroid-Hormone, T-Box-Domain-Proteins, T-Lymphocytes-Helper-Inducer

JAX Source

J Neuroimmunol 2009 Oct; 215(1-2):10-24.

Abstract

Th1/Th17 cells, secreting both IFNgamma and IL-17, are often associated with inflammatory pathology. We cloned and studied the cytokine phenotypes of MBP-specific, TCR-identical encephalitogenic CD4+ cells in relationship to Th1- and Th17-associated transcription factors T-bet and RORgammat. IFNgamma-producing cells could be sub-divided into those that are T-bet(+)/RORgammat(-) and those that are T-bet(+)/RORgammat(+). The latter comprises a spectrum of phenotypes, as defined by IL-17 production, and can be induced to up-regulate IL-23R with IL-12 or IL-23. The former, bona fide Th1 cells, lack IL-23R expression under all conditions. In vivo, T-bet(+)/RORgammat(-) and T-bet(+)/RORgammat(+) clones induce EAE equally well.